Lindsay D S, Toivio-Kinnucan M A, Blagburn B L
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519, USA.
Vet Parasitol. 1998 Jun 15;77(2-3):75-81. doi: 10.1016/s0304-4017(98)00099-5.
Decoquinate is an anticoccidial agent that inhibits respiration in the parasites mitochondrion. We examined human foreskin fibroblast cell cultures infected with the normally tissue cyst-less RH strain of Toxoplasma gondii and treated with decoquinate for evidence of tissue cyst induction and formation. Transmission electron microscopy observations demonstrated tissue cysts in decoquinate-treated cultures on days 3, 4, 5, and 6 after inoculation. Tissue cysts contained a tissue cyst wall that enclosed stages that resembled tachyzoites and stages that were structurally bradyzoites. Similar treatment of human foreskin fibroblast cells infected with tachyzoites of the TS-4 temperature-sensitive mutant of the RH strain did not result in production of tissue cysts.
