霍乱弧菌O1型外膜蛋白OmpU的特性分析
Characterization of outer membrane protein OmpU of Vibrio cholerae O1.
作者信息
Nakasone N, Iwanaga M
机构信息
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
出版信息
Infect Immun. 1998 Oct;66(10):4726-8. doi: 10.1128/IAI.66.10.4726-4728.1998.
Abstract
The outer membrane protein OmpU of Vibrio cholerae O1 strain 86B3 was characterized with reference to colonization of the intestine by the organism. The purified OmpU exhibited a pI of 3.6. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it migrated to 38, 32, and 110 kDa when the sample was heated at 100 degrees C for 2 min, 50 degrees C for 15 min, and room temperature for 30 min, respectively. The purified OmpU was not hemagglutinative. Anti-OmpU serum did not agglutinate strain 86B3 or other V. cholerae organisms. OmpU adhered to the brush border of the rabbit small intestine; adhesion of the organisms to the intestine treated in advance with OmpU was not inhibited. Treating the organisms in advance with anti-OmpU Fab did not inhibit adhesion to the intestine. These results obtained in vitro suggest that OmpU is not involved in the adhesion of V. cholerae to the intestinal epithelium.
摘要
参照霍乱弧菌O1菌株86B3在肠道中的定殖情况，对其外膜蛋白OmpU进行了表征。纯化后的OmpU的等电点为3.6。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中，当样品分别在100℃加热2分钟、50℃加热15分钟和室温下放置30分钟时，它分别迁移至38 kDa、32 kDa和110 kDa。纯化后的OmpU没有血凝活性。抗OmpU血清不会凝集86B3菌株或其他霍乱弧菌。OmpU粘附于兔小肠的刷状缘；预先用OmpU处理过的肠道对该菌的粘附没有受到抑制。预先用抗OmpU Fab处理该菌不会抑制其对肠道的粘附。这些体外实验结果表明，OmpU不参与霍乱弧菌对肠上皮的粘附。
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