Kokuba H, Imafuku S, Huang S, Aurelian L, Burnett J W
Virology/Immunology Laboratories, University of Maryland School of Medicine, Baltimore 21201, USA.
Br J Dermatol. 1998 Jun;138(6):952-64. doi: 10.1046/j.1365-2133.1998.02260.x.
A common form of erythema multiforme, herpes-associated erythema multiforme (HAEM), occurs following infection with herpes simplex virus (HSV). Here we report that HSV gene expression and the qualitative nature of the virus-specific T-cell responses are related to HAEM lesion development. Skin from HAEM lesions and 1-3 months healed HAEM lesional skin were positive for the viral DNA polymerase gene (Pol) by polymerase chain reaction. However, gene expression as determined by immunohistochemistry with Pol-specific antibody was seen only in HAEM lesions, suggesting that lesion development is associated with Pol gene expression. Similar HSV-specific T-cell lymphoproliferative responses were seen in peripheral blood mononuclear cells (PBMCs) from patients with acute or healed HAEM lesions or HSV lesions and from HSV-seropositive patients with unrelated inflammatory diseases. However, the T-cell receptor variable (V beta) chain repertoire of HSV-stimulated PBMCs obtained from HAEM lesions was altered; the prevalence of some families of variable chain (namely V beta 16 and V beta 19) was reduced, whereas the prevalence of others was increased (namely V beta 2 and V beta 7). V beta 2 cells were found in HAEM lesional skin positive for Pol antigen, suggesting that these cells home to viral antigen-positive skin.
一种常见的多形红斑形式，即疱疹相关性多形红斑（HAEM），发生在单纯疱疹病毒（HSV）感染之后。在此我们报告，HSV基因表达以及病毒特异性T细胞反应的性质与HAEM皮损的发展有关。通过聚合酶链反应，HAEM皮损处及愈合1 - 3个月的HAEM皮损皮肤中的病毒DNA聚合酶基因（Pol）呈阳性。然而，用Pol特异性抗体进行免疫组织化学检测确定的基因表达仅在HAEM皮损中可见，这表明皮损发展与Pol基因表达相关。在急性或愈合的HAEM皮损患者、HSV皮损患者以及患有无关炎症性疾病的HSV血清阳性患者的外周血单个核细胞（PBMC）中，观察到类似的HSV特异性T细胞增殖反应。然而，从HAEM皮损中获得的经HSV刺激的PBMC的T细胞受体可变（Vβ）链谱发生了改变；一些可变链家族（即Vβ16和Vβ19）的发生率降低，而其他家族（即Vβ2和Vβ7）的发生率增加。在Pol抗原呈阳性的HAEM皮损皮肤中发现了Vβ2细胞，这表明这些细胞归巢至病毒抗原阳性皮肤。
