人巨细胞病毒自然杀伤细胞诱饵基因UL18在体外和体内的转录

Transcription of the human cytomegalovirus natural killer decoy gene, UL18, in vitro and in vivo.

作者信息

Hassan-Walker A F, Cope A V, Griffiths P D, Emery V C

机构信息

Department of Virology, Royal Free Hospital School of Medicine, London, UK.

出版信息

J Gen Virol. 1998 Sep;79 ( Pt 9):2113-6. doi: 10.1099/0022-1317-79-9-2113.

DOI:10.1099/0022-1317-79-9-2113

PMID:9747719

Abstract

Multiplex RT-PCR analysis of human cytomegalovirus (HCMV) replication in human fibroblasts showed transcription of the natural killer (NK) cell decoy gene, UL18, from 72 h onwards. Transcription of glycoprotein B (gpUL55; a late gene) occurred from early time-points and peaked at 24 h post-infection. UL18 mRNA was also detected in the peripheral blood mononuclear cells of organ transplant recipients with HCMV viraemia, especially those with HCMV DNA virus loads greater than 10(5) genomes/ml whole blood. Thus, UL18 is produced via a low abundance transcript late during the infectious cycle at a time coincidental with the increased risk of NK cell lysis as a consequence of class I HLA down-regulation.

摘要

对人成纤维细胞中人类巨细胞病毒（HCMV）复制进行的多重逆转录聚合酶链反应（Multiplex RT-PCR）分析显示，自然杀伤（NK）细胞诱饵基因UL18从72小时起开始转录。糖蛋白B（gpUL55；一种晚期基因）在早期时间点开始转录，并在感染后24小时达到峰值。在患有HCMV病毒血症的器官移植受者的外周血单个核细胞中也检测到了UL18 mRNA，尤其是那些HCMV DNA病毒载量大于10⁵基因组/ml全血的受者。因此，UL18是在感染周期后期通过低丰度转录本产生的，此时正值由于I类HLA下调导致NK细胞裂解风险增加之际。