Frost F J, de la Cruz A A, Moss D M, Curry M, Calderon R L
Southwest Center for Managed Care Research, Albuquerque, New Mexico 87108, USA.
Epidemiol Infect. 1998 Aug;121(1):205-11. doi: 10.1017/s0950268898008991.
A seroprevalence survey was conducted using ELISA and Western blot (WB) assays for antibody to three Cryptosporidium antigens on 380 blood donors in Jackson County, Oregon. The purpose was to determine if either assay could detect serological evidence of an outbreak which occurred in Talent, Oregon 6 months earlier. The ELISA, which tested for combined IgG, IgA and IgM, and the WB, which tested separately for IgG and IgA, detected an almost twofold increase in serological response for persons who consumed Talent drinking water during the previous 11 months. The increases, however, were statistically significant (P < 0.05) only for the WB. The identification of serological evidence of infection, using sera collected 6 months after the end of the outbreak in a population not selected because of cryptosporidiosis-like illness, suggests that assays of Cryptosporidium-specific IgG and IgA may assist in estimating the magnitude of asymptomatic infections in the population.
采用酶联免疫吸附测定（ELISA）和蛋白质印迹法（WB）检测俄勒冈州杰克逊县380名献血者针对三种隐孢子虫抗原的抗体，开展了一项血清阳性率调查。目的是确定这两种检测方法能否检测到6个月前在俄勒冈州塔伦特发生的一次疫情的血清学证据。ELISA检测总IgG、IgA和IgM，WB分别检测IgG和IgA，结果显示，在过去11个月饮用塔伦特饮用水的人群中，血清学反应几乎增加了两倍。然而，只有WB检测的增加具有统计学意义（P < 0.05）。在疫情结束6个月后，从非因隐孢子虫病样疾病而入选的人群中采集血清，鉴定出感染的血清学证据，这表明检测隐孢子虫特异性IgG和IgA可能有助于估计人群中无症状感染的规模。
