Frost F J, Calderon R L, Muller T B, Curry M, Rodman J S, Moss D M, de la Cruz A A
Southwest Center for Managed Care Research, Albuquerque, New Mexico 87108, USA.
Epidemiol Infect. 1998 Aug;121(1):213-7. doi: 10.1017/s095026889800898x.
To estimate the duration of Cryptosporidium-specific antibody, a Western blot assay measured antibody in paired sera from 124 residents of Jackson County, Oregon collected 0.5 and 2.5 years after the end of an outbreak in Talent, Jackson County. The outcome measure was the intensity of antibody responses, (which may approximate to a titre), to 27-kDa and 15/17-kDa antigens. Intensity of response to the 27-kDa antigen(s) declined to 54% of the 1992 value while responses to a 15/17-kDa antigen(s) remained close to the initial values. Increasing age of the donor predicted higher intensity of antibody to the 15/17-kDa antigen(s) in both the initial (P = 0.004) and follow-up (P = 0.038) surveys. No relationship was observed between age and antibody intensity for the 27-kDa antigen(s) during either survey (P > 0.10). Both the initial and follow-up surveys showed significant elevations in antibody intensity for Talent residents, possibly indicating a high endemic rate of infection/re-infection or high levels of chronic infection.
为了估计隐孢子虫特异性抗体的持续时间，采用蛋白质印迹法检测了俄勒冈州杰克逊县124名居民配对血清中的抗体，这些血清分别在杰克逊县塔伦特疫情结束后0.5年和2.5年采集。结果指标是对27 kDa和15/17 kDa抗原的抗体反应强度（可能近似于滴度）。对27 kDa抗原的反应强度降至1992年值的54%，而对15/17 kDa抗原的反应强度仍接近初始值。在初始调查（P = 0.004）和随访调查（P = 0.038）中，供体年龄越大，对15/17 kDa抗原的抗体强度越高。在任何一次调查中，均未观察到年龄与27 kDa抗原抗体强度之间的关系（P > 0.10）。初始调查和随访调查均显示，塔伦特居民的抗体强度显著升高，这可能表明感染/再感染的流行率较高或慢性感染水平较高。
