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甘蔗杆状杆状病毒启动子在单子叶植物和双子叶植物中均具有活性。

The sugarcane bacilliform badnavirus promoter is active in both monocots and dicots.

作者信息

Tzafrir I, Torbert K A, Lockhart B E, Somers D A, Olszewski N E

机构信息

Department of Plant Biology, University of Minnesota, St. Paul 55108, USA.

出版信息

Plant Mol Biol. 1998 Oct;38(3):347-56. doi: 10.1023/a:1006075415686.

Abstract

Regions of the sugarcane bacilliform badnavirus genome were tested for promoter activity. The genomic region spanning nucleotides 5999-7420 was shown to possess promoter activity as exemplified by its ability to drive the expression of the coding region of the uidA gene of Escherichia coli, in both Avena sativa and Arabidopsis thaliana. In A. sativa, the promoter was active in all organs examined and, with the exception of the anthers where the expression was localized, this activity was constitutive. In A. thaliana, the promoter activity was constitutive in the rosette leaf, stem, stamen, and root and limited primarily to vascular tissue in the sepal and the silique. The transgene was inherited and active in progeny plants of both A. sativa and A. thaliana.

摘要

对甘蔗杆状杆状病毒基因组的区域进行了启动子活性测试。跨越核苷酸5999 - 7420的基因组区域显示具有启动子活性,例如它能够驱动大肠杆菌uidA基因编码区在燕麦和拟南芥中的表达。在燕麦中,该启动子在所检测的所有器官中均有活性,除了花药中表达局限于特定部位外,这种活性是组成型的。在拟南芥中,启动子活性在莲座叶、茎、雄蕊和根中是组成型的,并且主要局限于萼片和角果的维管组织。转基因在燕麦和拟南芥的子代植株中都能遗传并具有活性。

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