Molloy J B, Bowles P M, Jeston P J, Bruyeres A G, Bowden J M, Bock R E, Jorgensen W K, Blight G W, Dalgliesh R J
Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
Parasitol Res. 1998 Aug;84(8):651-6. doi: 10.1007/s004360050465.
Monoclonal antibodies, directed against a 58-kDa Babesia bigemina merozoite antigen that reacted strongly with immune sera from experimentally and naturally infected cattle in Western blots, were used to develop a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). As based on the testing of 70 antibody-positive sera from experimentally infected cattle and 166 antibody-negative sera collected in non-endemic areas of Australia, the sensitivity and specificity of the ELISA were 95.7% and 97.0%, respectively. In sequential sera collected from six calves during the course of experimental B. bigemina infections the ELISA detected seroconversion at about 10 days post-inoculation. The specificity of the ELISA was not affected by the presence of antibodies to B. bovis, Anaplasma marginale or Theileria buffeli. In 42 sera from cattle experimentally infected with B. bovis but negative for B. bigemina the specificity of the ELISA was 95.2%. The use of a competitive-inhibition ELISA format detecting only antibody directed against a single epitope on the 58-kDa antigen appears to have overcome many of the specificity problems that have plagued serological tests for B. bigemina in the past. The test should be useful for epidemiology studies, particularly in areas where B. bovis and B. bigemina have overlapping distributions.
针对一种58 kDa的双芽巴贝斯虫裂殖子抗原的单克隆抗体，该抗原在蛋白质印迹法中与来自实验感染和自然感染牛的免疫血清发生强烈反应，用于开发一种竞争抑制酶联免疫吸附测定（ELISA）。基于对来自实验感染牛的70份抗体阳性血清和在澳大利亚非流行地区收集的166份抗体阴性血清的检测，该ELISA的敏感性和特异性分别为95.7%和97.0%。在实验性双芽巴贝斯虫感染过程中从六头小牛采集的系列血清中，ELISA在接种后约10天检测到血清转化。ELISA的特异性不受牛巴贝斯虫、边缘无浆体或布氏泰勒虫抗体存在的影响。在42份来自实验感染牛巴贝斯虫但双芽巴贝斯虫阴性的血清中，ELISA的特异性为95.2%。使用仅检测针对58 kDa抗原上单个表位的抗体的竞争抑制ELISA形式似乎克服了过去困扰双芽巴贝斯虫血清学检测的许多特异性问题。该检测方法应有助于流行病学研究，特别是在牛巴贝斯虫和双芽巴贝斯虫分布重叠的地区。
