牛肺线虫（胎生网尾线虫）幼虫体外培养方法的改进。
Improved in vitro cultivation of larvae of the bovine lungworm Dictyocaulus viviparus.
作者信息
von Samson-Himmelstjerna G, Rickling S, Schnieder T
机构信息
Institute of Parasitology, School of Veterinary Medicine, Hannover, Germany.
出版信息
Parasitol Res. 1998 Aug;84(8):682-4. doi: 10.1007/s004360050469.
Parasitic larvae of Dictyocaulus viviparus are of major importance for the development of immunity in cattle. The conditions for in vitro cultivation of D. viviparus larvae as well as their morphology have thus far been only poorly investigated. Exsheathed larvae were cultivated in vitro in RPMI-1640 (Gibco-BRL, pH 7.2) containing 20% newborn Calf serum, 200 U Moronal/ml, and 200 U penicillin/streptomycin/ml. Incubation was performed at 39.5 degrees C at 0, 5%, 10%, and 20% CO2. Average development rates to third-moult (3 M) or fourth-stage (L4) larvae at 5% CO2 incubation were 8.33% (SD +/- 7.76%), 22.52% (SD +/- 13.09%) at 10% CO2, and 38.01% (SD +/- 15.63%) at 20% CO2. These differences were statistically significant. Some morphological features of these larvae are described.
胎生网尾线虫的寄生幼虫对牛免疫力的发展至关重要。迄今为止，对胎生网尾线虫幼虫的体外培养条件及其形态的研究还很有限。将脱鞘幼虫在含有20%新生牛血清、200 U促肾上腺皮质激素/毫升和200 U青霉素/链霉素/毫升的RPMI - 1640（Gibco - BRL，pH 7.2）中进行体外培养。在39.5摄氏度、0%、5%、10%和20%二氧化碳条件下进行孵育。在5%二氧化碳孵育条件下发育至第三次蜕皮（3M）或第四阶段（L4）幼虫的平均发育率分别为8.33%（标准差±7.76%），在10%二氧化碳条件下为22.52%（标准差±13.09%），在20%二氧化碳条件下为38.01%（标准差±15.63%）。这些差异具有统计学意义。文中描述了这些幼虫的一些形态特征。
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