Smit-McBride Z, Mattapallil J J, Villinger F, Ansari A A, Dandekar S
Department of Internal Medicine, University of California at Davis 95616, USA.
J Med Primatol. 1998 Apr-Jun;27(2-3):129-40. doi: 10.1111/j.1600-0684.1998.tb00237.x.
Isolated intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) from jejunum of SIV infected animals were examined for alterations in basal cytokine expression by RT-PCR. Remarkable changes in IFNgamma and IL-10 RNA levels were observed in IEL and LPL in SIV infection while IL-4 and IL-2 RNA levels remained unaltered. In addition, the CD4+ and CD8+ LPL were examined for intracellular cytokine production following mitogenic activation by flowcytometry. Both CD4+ and CD8+ T lymphocytes in intestinal mucosa retained the potential to produce IFNgamma in response to mitogenic stimulation in vitro, without a remarkable change in IL-4 production. The dominant IFNgamma cytokine response could be one of the major contributing factors in SIV associated enteropathy.
通过逆转录聚合酶链反应（RT-PCR）检测来自感染猴免疫缺陷病毒（SIV）动物空肠的孤立上皮内淋巴细胞（IEL）和固有层淋巴细胞（LPL），以观察基础细胞因子表达的变化。在SIV感染的IEL和LPL中观察到γ干扰素（IFNγ）和白细胞介素10（IL-10）RNA水平有显著变化，而IL-4和IL-2 RNA水平保持不变。此外，通过流式细胞术检测有丝分裂原激活后CD4⁺和CD8⁺ LPL的细胞内细胞因子产生情况。肠道黏膜中的CD4⁺和CD8⁺ T淋巴细胞在体外对有丝分裂原刺激均保留产生IFNγ的潜力，IL-4产生无显著变化。占主导地位的IFNγ细胞因子反应可能是SIV相关肠病的主要促成因素之一。
