Ndd是一种能破坏大肠杆菌类核的噬菌体T4蛋白，它具有DNA结合活性。
Ndd, the bacteriophage T4 protein that disrupts the Escherichia coli nucleoid, has a DNA binding activity.
作者信息
Bouet J Y, Krisch H M, Louarn J M
机构信息
Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Toulouse, France.
出版信息
J Bacteriol. 1998 Oct;180(19):5227-30. doi: 10.1128/JB.180.19.5227-5230.1998.
Abstract
Early in a bacteriophage T4 infection, the phage ndd gene causes the rapid destruction of the structure of the Escherichia coli nucleoid. Even at very low levels, the Ndd protein is extremely toxic to cells. In uninfected E. coli, overexpression of the cloned ndd gene induces disruption of the nucleoid that is indistinguishable from that observed after T4 infection. A preliminary characterization of this protein indicates that it has a double-stranded DNA binding activity with a preference for bacterial DNA rather than phage T4 DNA. The targets of Ndd action may be the chromosomal sequences that determine the structure of the nucleoid.
摘要
在噬菌体T4感染早期，噬菌体ndd基因会导致大肠杆菌类核结构迅速被破坏。即使在极低水平下，Ndd蛋白对细胞也极具毒性。在未感染的大肠杆菌中，克隆的ndd基因的过表达会诱导类核的破坏，这与T4感染后观察到的情况无法区分。对该蛋白的初步表征表明，它具有双链DNA结合活性，更倾向于结合细菌DNA而非噬菌体T4 DNA。Ndd作用的靶点可能是决定类核结构的染色体序列。
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