建立更好的感染环境：感染期间早期噬菌体T4基因的过表达为噬菌体产生了更有利的tRNA库。

Setting Up a Better Infection: Overexpression of the Early Bacteriophage T4 Gene During Infection Results in a More Favorable tRNA Pool for the Phage.

作者信息

Son Bokyung, Patterson-West Jennifer, Thompson Christine O, Iben James R, Hinton Deborah M

机构信息

Gene Expression and Regulation Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Molecular Genomics Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.

出版信息

Phage (New Rochelle). 2022 Sep 1;3(3):141-152. doi: 10.1089/phage.2022.0023. Epub 2022 Sep 19.

DOI:10.1089/phage.2022.0023

PMID:36196375

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9527043/

Abstract

BACKGROUND

Although many bacteriophage T4 early genes are nonessential with unknown functions, they are believed to aid in the takeover of the host. Understanding the functions of these genes could be helpful to develop novel antibacterial strategies. MotB, encoded by a previously uncharacterized T4 early gene, is a DNA-binding protein that compacts the host nucleoid and alters host gene expression.

METHODS

MotB structure was predicted by AlphaFold 2. RNA-seq and mass spectrometry (MS) analyses were performed to determine RNA and protein changes when was overexpressed in BL21(DE3) ±5 min T4 infection.

RESULTS

MotB structure is predicted to be a two-domain protein with N-terminal Kyprides-Onzonis-Woese and C-terminal oligonucleotide/oligosaccharide-fold domains. In B, overexpression during infection does not affect T4 RNAs, but affects the expression of host genes, including the downregulation of 21 of the 84 chargeable host tRNAs. Many of these tRNAs are used less frequently by T4 or have a counterpart encoded within the T4 genome. The MS analyses indicate that the levels of multiple T4 proteins are changed by overexpression.

CONCLUSION

Our results suggest that in this B host, is involved in establishing a more favorable tRNA pool for the phage during infection.

摘要

背景

尽管许多噬菌体T4早期基因并非必需且功能未知，但它们被认为有助于接管宿主。了解这些基因的功能可能有助于开发新的抗菌策略。MotB由一个以前未被表征的T4早期基因编码，是一种DNA结合蛋白，可使宿主类核浓缩并改变宿主基因表达。

方法

通过AlphaFold 2预测MotB结构。进行RNA测序和质谱（MS）分析，以确定MotB在BL21(DE3)中过表达且T4感染±5分钟时RNA和蛋白质的变化。

结果

MotB结构预计为一种双结构域蛋白，具有N端的基普里兹-翁佐尼斯-沃斯结构域和C端的寡核苷酸/寡糖折叠结构域。在大肠杆菌B中，感染期间MotB的过表达不影响T4 RNA，但影响宿主基因的表达，包括84种可充电宿主tRNA中的21种下调。这些tRNA中的许多在T4中使用频率较低，或者在T4基因组中有对应的编码。MS分析表明，MotB的过表达会改变多种T4蛋白的水平。