Schambony A, Gentzel M, Wolfes H, Raida M, Neumann U, Töpfer-Petersen E
Institut für Reproduktionsmedizin, Tierärztliche Hochschule, Bünteweg 15, D-30559 Hannover, Germany.
Biochim Biophys Acta. 1998 Sep 8;1387(1-2):206-16. doi: 10.1016/s0167-4838(98)00122-8.
Although originally described in the male rodent genital tract, cysteine-rich secretory proteins (CRISPs) are expressed in a variety of mammalian tissue and cell types. The proteins of the male genital tract have been observed associated to spermatozoa and are believed to play a role in mammalian fertilization. Here we describe the identification and primary structure of the first equine member of the CRISP family. Equine CRISP-3 is transcribed and expressed in the stallion salivary gland, in the ampulla and the seminal vesicle. It displays all 16 conserved cysteine residues and shows 82% homology to human and 78% to guinea pig CRISP-2 (AA1, TPX 1) and 77% to human CRISP-3. In contrast to other mammalia, in the horse CRISP-3 is synthesized in great amounts in the accessory sexual glands, ampulla and seminal vesicle, thus allowing the isolation of equine CRISP-3 in amounts suitable for biochemical, physiological and structural studies from stallion seminal plasma.
尽管富含半胱氨酸的分泌蛋白（CRISPs）最初是在雄性啮齿动物生殖道中被描述的，但它们在多种哺乳动物组织和细胞类型中都有表达。已观察到雄性生殖道中的这些蛋白质与精子相关，并被认为在哺乳动物受精过程中发挥作用。在此，我们描述了CRISP家族首个马属成员的鉴定及一级结构。马属CRISP-3在种马的唾液腺、壶腹和精囊中进行转录和表达。它具有全部16个保守的半胱氨酸残基，与人类CRISP-2的同源性为82%，与豚鼠CRISP-2（AA1，TPX 1）的同源性为78%，与人类CRISP-3的同源性为77%。与其他哺乳动物不同，在马中，CRISP-3在附属性腺、壶腹和精囊中大量合成，因此能够从种马精浆中分离出数量适合进行生化、生理和结构研究的马属CRISP-3。
