Sewchand L, Canham P B
Can J Physiol Pharmacol. 1976 Aug;54(4):437-42. doi: 10.1139/y76-062.
A study was made of rouleaux formation between erythrocytes of different species when suspended in Ringer solution with polyvinylpyrrolidone added (PVP with M.W 360 000, 4.0 G/L). PVP was shown previously to be a suitable asymmetric macromolecule for promoting rouleaux formation. For the present study fresh samples of blood were obtained from humans, cats, rats, mice, dogs, rabbits and guinea pigs. Initally homogenous populations of cells were allowed to form rouleaux in the microscope chamber for the purpose of determining the average cellular dimensions for each species. Subsequently red cells from two species at a time were mixed in equal proportions to assess the degree of preference for the cells of one species to form rouleaux among themselves rather than with cells of the other species. The sequencing of the cells in the mixed rouleaux which formed on the coverslip was determined from photomicrographs, using the previously determined knowledge of the cellular dimensions. Although the visual impression was that the rouleaux were composed of a random selection of cells from the mixed population, a preferencnstrated statisically in nine of the ten combinations tested. Only the rat-mouse mixture of cells was excepted, perhaps because these animals are closely related members of the same family.
进行了一项研究，观察不同物种的红细胞悬浮于添加了聚乙烯吡咯烷酮（分子量360000，4.0g/L）的林格溶液中时的缗钱状形成情况。聚乙烯吡咯烷酮先前已被证明是一种适合促进缗钱状形成的不对称大分子。在本研究中，从人类、猫、大鼠、小鼠、狗、兔子和豚鼠获取新鲜血液样本。最初，让均匀的细胞群体在显微镜载物台上形成缗钱状，以确定每个物种的平均细胞尺寸。随后，每次将两个物种的红细胞按等比例混合，以评估一个物种的细胞相较于与另一个物种的细胞更倾向于彼此形成缗钱状的程度。利用先前确定的细胞尺寸知识，通过显微照片确定在盖玻片上形成的混合缗钱状中细胞的排列顺序。尽管视觉上的印象是缗钱状由混合群体中随机选择的细胞组成，但在测试的十种组合中的九种中，统计学上显示出偏好。只有大鼠 - 小鼠细胞混合物除外，可能是因为这两种动物是同一家族关系密切的成员。
