Kvasnicka J, Rypácková B, Calda P, Hájek Z
Department of Clinical Haematology, General University Hospital, Prague, Czech Republic.
Sb Lek. 1998;99(1):13-20.
Examination of RhD genotype (Rhesus D gene) from amniotic cells (or chorionic villi cells) by PCR amplification of DNA can be done at early stage of pregnancy. Due to some missing exons in the Rh partial D variant (e.g. DVI), it is necessary to use different PCR systems to get relevant results. The localization of primers in our three PCR systems is on the different exons (10, 7, and 4 + 5). The advantage of PCR technique is prenatal detection of RhD of fetus from nonerythrocytes suspension (e.g. from an amnion fluid cell sediment) in comparison to a "standard" haemagglutination serological technique which uses blood erythrocytes only. The possibility of this technique to distinguished the heterozygous (D/d) or homozygous (D/D) fathers can help the clinicians in decisions about the management of further prevention of the hemolytic disease of newborn.
通过对羊水细胞（或绒毛膜绒毛细胞）的DNA进行PCR扩增来检测RhD基因型（恒河猴D基因），可在妊娠早期进行。由于Rh部分D变异体（如DVI）中存在一些缺失外显子，因此有必要使用不同的PCR系统来获得相关结果。我们三个PCR系统中引物的定位在不同的外显子上（10、7以及4 + 5）。与仅使用血液红细胞的“标准”血凝血清学技术相比，PCR技术的优势在于可从非红细胞悬液（如羊水细胞沉淀物）中对胎儿的RhD进行产前检测。该技术区分杂合子（D/d）或纯合子（D/D）父亲的可能性，有助于临床医生决定进一步预防新生儿溶血病的管理措施。
