Phuntsok T, Froetschel M A, Amos H E, Zheng M, Huang Y W
Department of Animal and Dairy Science, University of Georgia, Athens 30602, USA.
J Dairy Sci. 1998 Aug;81(8):2193-203. doi: 10.3168/jds.S0022-0302(98)75798-4.
A 4 x 4 Latin square experiment was conducted to examine abomasal passage of biogenic amines in steers fed silage and their related effects on intake, digestibility, and digestive function. Thirty percent of the dry matter (DM) in the diets consisted of alfalfa forage, which was fed as either hay or silage. The DM from alfalfa silage DM was substituted at 0, 33, 67, and 100% for DM from alfalfa hay and was fed to four ruminally and abomasally cannulated steers. The roughage component of the diet constituted 50% of the DM and consisted of 60% alfalfa silage or hay and 40% tropical corn silage. The concentrate was composed mainly of ground corn. The concentrations of putrescine and cadaverine in abomasal digesta increased as alfalfa silage in the diet increased. Abomasal recovery of biogenic amines, a product of their concentration in abomasal digesta and the passage of DM through the abomasum, was negatively correlated with intake. Abomasal recovery of most amines was 5 to 20% of intake. Abomasal recovery of cadaverine was correlated with depressed intake. Total DM intake was reduced 8.3 to 25.8% as the proportion of alfalfa silage in the diet increased. Frequency of reticular contractions, intake, ruminal DM digestibility, ruminal outflow, volatile fatty acids, and total tract DM digestibility decreased in steers fed diets that contained more alfalfa silage. Ruminal fluid pH and NH3 concentration increased in steers fed more alfalfa silage; however, mass and the DM percentage of ruminal contents decreased linearly. Postprandial insulin concentrations were quadratically related to the proportion of alfalfa hay or silage in the diet. Intraruminal metabolism of biogenic amines is extensive based on the relatively low quantities recovered in abomasal digesta; however, the amounts recovered in abomasal digesta were related to intake depression and associated physiological effects.
进行了一项4×4拉丁方试验，以研究饲喂青贮饲料的阉牛皱胃中生物胺的通过情况及其对采食量、消化率和消化功能的相关影响。日粮中30%的干物质（DM）由苜蓿饲料组成，以干草或青贮饲料的形式饲喂。苜蓿青贮饲料的DM分别以0、33、67和100%替代苜蓿干草的DM，并喂给4头安装了瘤胃和皱胃瘘管的阉牛。日粮的粗饲料部分占DM的50%，由60%的苜蓿青贮饲料或干草和40%的热带玉米青贮饲料组成。精料主要由玉米粉组成。随着日粮中苜蓿青贮饲料的增加，皱胃消化物中腐胺和尸胺的浓度升高。生物胺在皱胃中的回收率（其在皱胃消化物中的浓度与DM通过皱胃的量的乘积）与采食量呈负相关。大多数胺类在皱胃中的回收率为采食量的5%至20%。尸胺在皱胃中的回收率与采食量降低相关。随着日粮中苜蓿青贮饲料比例的增加，总DM采食量降低了8.3%至25.8%。饲喂含有更多苜蓿青贮饲料日粮的阉牛，其网胃收缩频率、采食量、瘤胃DM消化率、瘤胃流出物、挥发性脂肪酸和全消化道DM消化率均降低。饲喂更多苜蓿青贮饲料的阉牛，其瘤胃液pH值和NH3浓度升高；然而，瘤胃内容物的质量和DM百分比呈线性下降。餐后胰岛素浓度与日粮中苜蓿干草或青贮饲料的比例呈二次相关。基于在皱胃消化物中回收的相对少量，生物胺在瘤胃内的代谢是广泛的；然而，在皱胃消化物中回收的量与采食量降低及相关生理效应有关。
