Olano C, Rodriguez A M, Michel J M, Méndez C, Raynal M C, Salas J A
Departamento de Biología Funcional, Instituto Universitario de Biotecnologia de Asturias (IUBA-CSIC), Universidad de Oviedo, Spain.
Mol Gen Genet. 1998 Aug;259(3):299-308. doi: 10.1007/s004380050816.
A 6-kb region from the chromosome of Streptomyces antibioticus, an oleandomycin producer, was cloned and sequenced. This region was located between the 3' end of the gene encoding the third subunit of the oleandomycin type I polyketide synthase and the oleP and oleB genes, which encode a cytochrome P450 monooxygenase and an oleandomycin resistance gene, respectively. Analysis of the nucleotide sequence revealed the presence of five genes encoding a cytochrome P450-like protein (oleP1), two glycosyltransferases (oleG1 and oleG2) involved in the transfer of the two 6-deoxysugars (L-oleandrose and D-desosamine) to the oleandomycin macrolactone ring, a methyltransferase (oleM1), and a gene (oleY) of unknown function. Insertional inactivation of this region by gene disruption generated an oleandomycin non-producing mutant which accumulated a compound that, according to mass spectrometry analysis, could correspond to the oleandomycin macrolactone ring (oleandolide), suggesting that the mutation affects oleandrosyl glycosyltransferase.
从产竹桃霉素的抗生链霉菌染色体中克隆并测序了一段6 kb的区域。该区域位于编码竹桃霉素I型聚酮合酶第三亚基的基因3'端与oleP和oleB基因之间，oleP和oleB基因分别编码一种细胞色素P450单加氧酶和一个竹桃霉素抗性基因。核苷酸序列分析显示存在五个基因，分别编码一种细胞色素P450样蛋白（oleP1）、两种参与将两种6-脱氧糖（L-夹竹桃糖和D-去氧氨基糖）转移至竹桃霉素大环内酯环的糖基转移酶（oleG1和oleG2）、一种甲基转移酶（oleM1）以及一个功能未知的基因（oleY）。通过基因破坏对该区域进行插入失活产生了一个不产竹桃霉素的突变体，该突变体积聚了一种化合物，根据质谱分析，该化合物可能对应于竹桃霉素大环内酯环（夹竹桃醇内酯），这表明该突变影响夹竹桃糖基糖基转移酶。
