Rodriguez A M, Olano C, Méndez C, Hutchinson C R, Salas J A
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
FEMS Microbiol Lett. 1995 Mar 15;127(1-2):117-20. doi: 10.1111/j.1574-6968.1995.tb07459.x.
A cosmid clone from an oleandomycin producer, Streptomyces antibioticus, contains a large open reading frame encoding a type I polyketide synthase subunit and an oleandomycin resistance gene (oleB). Sequencing of a 1.4-kb DNA fragment adjacent to oleB revealed the existence of an open reading frame (oleP) encoding a protein similar to several cytochrome P450 monooxygenases from different sources, including the products of the eryF and eryK genes from Saccharopolyspora erythraea that participate in erythromycin biosynthesis. The oleP gene was expressed in Escherichia coli as a fusion protein to a maltose-binding protein. Using polyclonal antibodies against this fusion protein it was observed that the synthesis of the cytochrome P450 was in parallel to that of oleandomycin. The cytochrome P450 encoded by the oleP gene could be responsible for the epoxidation of carbon 8 of the oleandomycin lactone ring.
来自产竹桃霉素的抗生素链霉菌的一个黏粒克隆，含有一个编码I型聚酮合酶亚基的大的开放阅读框和一个竹桃霉素抗性基因（oleB）。对与oleB相邻的一个1.4kb DNA片段进行测序，发现存在一个开放阅读框（oleP），其编码的一种蛋白质与来自不同来源的几种细胞色素P450单加氧酶相似，包括参与红霉素生物合成的来自糖多孢红霉菌的eryF和eryK基因的产物。oleP基因在大肠杆菌中作为与麦芽糖结合蛋白的融合蛋白表达。使用针对该融合蛋白的多克隆抗体，观察到细胞色素P450的合成与竹桃霉素的合成平行。oleP基因编码的细胞色素P450可能负责竹桃霉素内酯环C8位的环氧化反应。
