Hawkes R, Faulkner-Jones B, Tam P, Tan S S
Department of Anatomy and Neuroscience Research Group, Faculty of Medicine, The University of Calgary, Alberta, Canada.
Eur J Neurosci. 1998 Feb;10(2):790-3. doi: 10.1046/j.1460-9568.1998.00085.x.
The cerebellar cortex is subdivided into an elaborate, stereotyped array of transverse zones and parasagittal stripes. It has been speculated that (i) all Purkinje cells derive from 10 to 20 precursors allocated early in embryogenesis and (ii) that pattern formation is based on cell lineage restriction in the founder pool. These hypotheses have been tested by clonal analysis of embryonic stem cell chimeras. Neither speculation is supported: the analysis suggests that Purkinje cells derive from a founder population of > 102 precursors, and that neither cerebellar transverse developmental boundaries nor parasagittal stripes have a clonal origin. We conclude that early lineage restriction plays no role in cerebellar pattern formation.
小脑皮质被细分为一系列复杂的、模式化的横向区域和矢状旁条纹。据推测，（i）所有浦肯野细胞均来源于胚胎发育早期分配的10至20个前体细胞，以及（ii）模式形成基于奠基细胞库中的细胞谱系限制。这些假设已通过对胚胎干细胞嵌合体的克隆分析进行了验证。两种推测均未得到支持：分析表明浦肯野细胞来源于超过102个前体细胞的奠基群体，并且小脑横向发育边界和矢状旁条纹均无克隆起源。我们得出结论，早期谱系限制在小脑模式形成中不起作用。
