Martínez-Bueno M, Valdivia E, Martín I, Quirantes R, Maqueda M
Departamento de Microbiología, Facultad Ciencias, University of Granada, Spain.
Lett Appl Microbiol. 1998 Sep;27(3):125-9. doi: 10.1046/j.1472-765x.1998.t01-1-00417.x.
The sexpheromone system of Enterococcus faecalis is a form of bacterial conjugation that plays an important role in the horizontal dissemination of genes. The ecological significance of 'sexual' plasmids is to permit a rapid mobilization of genes of interest for the species (e.g. those encoding haemolysins, bacteriocins or antibiotic resistance). The physical mapping of pMB1-1, a conjugative plasmid of Ent. faecalis that responds to cCF10 pheromone, has been undertaken. By means of hybridization with conserved sequences of pCF10 plasmid, the regions harbouring the genes responsible for the pheromone inhibitor and the aggregation and exclusion proteins of this plasmid have been identified. The results demonstrated that plasmids pMB1-1 and pCF10 only show homology in the region involved in the conjugative response, suggesting that this region may be transferred in an independent way to that of the rest of the plasmid.
粪肠球菌的性信息素系统是一种细菌接合形式，在基因的水平传播中发挥着重要作用。“性”质粒的生态意义在于允许快速调动该物种感兴趣的基因（例如那些编码溶血素、细菌素或抗生素抗性的基因）。已对粪肠球菌的一种对cCF10信息素作出反应的接合性质粒pMB1-1进行了物理图谱绘制。通过与pCF10质粒的保守序列杂交，已鉴定出该质粒中负责信息素抑制剂以及聚集和排斥蛋白的基因所在区域。结果表明，质粒pMB1-1和pCF10仅在参与接合反应的区域显示出同源性，这表明该区域可能以独立于质粒其余部分的方式进行转移。
