粪肠球菌S-48中pMB1和pMB2质粒特异性性信息素的纯化

Purification of sex pheromones specific for pMB1 and pMB2 plasmids of Enterococcus faecalis S-48.

作者信息

Quirantes R, Gálvez A, Valdivia E, Martín I, Martínez-Bueno M, Méndez E, Maqueda M

机构信息

Department of Microbiology, Faculty of Sciences, University of Granada, Spain.

出版信息

Can J Microbiol. 1995 Jul;41(7):629-32. doi: 10.1139/m95-084.

DOI:10.1139/m95-084

PMID:7641145

Abstract

The strain Enterococcus faecalis S-48 carries two large conjugative plasmids (pMB1 and pMB2) encoding for antagonistic substances. The pheromone response of these two plasmids was established by purifying the corresponding sex pheromones, using conventional reversed-phase columns. Plasmid pMB1 responds to pheromone cCF10. Plasmid pMB2 responds to a pheromone with an amino acid sequence identical to that of cPD1 (Phe-Leu-Val-Met-Phe-Leu-Ser-Gly). The two pheromone-responding plasmids coexist in a stable fashion in the wild-type strain E. faecalis S-48.

摘要

粪肠球菌S-48菌株携带两个编码拮抗物质的大型接合质粒（pMB1和pMB2）。通过使用常规反相柱纯化相应的性信息素，确定了这两个质粒的信息素反应。质粒pMB1对信息素cCF10有反应。质粒pMB2对一种氨基酸序列与cPD1（苯丙氨酸-亮氨酸-缬氨酸-甲硫氨酸-苯丙氨酸-亮氨酸-丝氨酸-甘氨酸）相同的信息素有反应。这两个对信息素产生反应的质粒在野生型粪肠球菌S-48菌株中以稳定的方式共存。