Mukai T, Kaneko S, Ohori H
School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Japan.
Lett Appl Microbiol. 1998 Sep;27(3):130-4. doi: 10.1046/j.1472-765x.1998.00418.x.
The carbohydrate-binding activity of Lactobacillus reuteri was studied by haemagglutination (HA), HA inhibition and thin layer chromatography (TLC) overlay assays. Three of the six Lact. reuteri strains examined showed HA activity. Two strains (JCM1081 and JCM1112T) agglutinated neuraminidase-treated, but not untreated, erythrocytes. Strain JCM2762 agglutinated both treated and untreated erythrocytes. The HA activity of JCM 1081 was inhibited by galactose, lactose, methyl beta-galactoside and asialoglycophorin A. Among 12 glycosphingolipids, TLC overlay assay showed that JCM1081 strongly bound to asialo-GM1. These results indicated that JCM1081 bound to the beta-galactosyl residues of the non-reducing terminal of sugar chains of glycoconjugates. The carbohydrate-binding ability of JCM1081 may be responsible for the adhesion of this strain to the mucosal surface of the intestine.
通过血细胞凝集（HA）、HA抑制和薄层色谱（TLC）覆盖分析研究了罗伊氏乳杆菌的碳水化合物结合活性。所检测的6株罗伊氏乳杆菌中有3株显示出HA活性。两株菌（JCM1081和JCM1112T）能凝集经神经氨酸酶处理但未经处理的红细胞。JCM2762菌株能凝集经处理和未经处理的红细胞。半乳糖、乳糖、β-甲基半乳糖苷和去唾液酸糖蛋白A可抑制JCM 1081的HA活性。在12种糖鞘脂中，TLC覆盖分析表明JCM1081与去唾液酸GM1强烈结合。这些结果表明JCM1081与糖缀合物糖链非还原末端的β-半乳糖基残基结合。JCM1081的碳水化合物结合能力可能是该菌株黏附于肠道黏膜表面的原因。
