大肠杆菌O157血清学：人抗体与牛血清白蛋白结合导致酶联免疫吸附测定结果出现假阳性
Escherichia coli O157 serology: false-positive ELISA results caused by human antibodies binding to bovine serum albumin.
作者信息
Chart H, Evans J, Chalmers R M, Salmon R L
机构信息
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
出版信息
Lett Appl Microbiol. 1998 Aug;27(2):76-8. doi: 10.1046/j.1472-765x.1998.00396.x.
Abstract
An analysis of farm workers and rural dwellers for serum antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157 detected sera with antibodies binding to bovine serum albumin (BSA) by ELISA. These antibodies were not specific for BSA when examined by immunoblotting, and the ELISA values were reduced to a background level when plates were blocked with normal rabbit serum.
摘要
对农场工人和农村居民血清中抗大肠杆菌O157脂多糖（LPS）抗体的分析发现，通过酶联免疫吸附测定（ELISA）检测到血清中存在与牛血清白蛋白（BSA）结合的抗体。通过免疫印迹法检测时，这些抗体对BSA不具有特异性，并且当用正常兔血清封闭酶标板时，ELISA值降至背景水平。
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