血清学诊断在与大肠杆菌O157:H7相关的托儿所疫情回顾性调查中的应用
The use of serodiagnosis in the retrospective investigation of a nursery outbreak associated with Escherichia coli O157:H7.
作者信息
Cheasty T, Robertson R, Chart H, Mannion P, Syed Q, Garvey R, Rowe B
机构信息
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
出版信息
J Clin Pathol. 1998 Jul;51(7):498-501. doi: 10.1136/jcp.51.7.498.
AIMS
To use serology to investigate an outbreak of verocytotoxin (VT) producing Escherichia coli O157 in a hospital nursery, following the detection of faecal E coli O157 (phage type 49) producing VT type 2.
METHODS
ELISA and immunoblotting techniques, based on lipopolysaccharide (LPS) purified from E coli O157; diagnostic bacteriology; serotyping and phage typing; DNA probes for VT.
RESULTS
29 of 126 sera contained antibodies to the LPS of E coli O157: 10 were from children, three were from staff, and 11 were from hospital kitchen staff. Five parents of children attending the nursery were antibody positive. Sixty four sera from other hospital staff and controls did not contain antibodies to the LPS of E coli O157.
CONCLUSIONS
Serology detected evidence of infection with E coli O157 in 23% of sera examined. By bacteriology alone, only a single case of infection with E coli O157 would have been detected. Serology is valuable in providing evidence of infection with E coli O157.
目的
在检测到产2型志贺毒素的粪便大肠杆菌O157（噬菌体分型49）后，采用血清学方法调查一家医院托儿所中由产志贺毒素（VT）的大肠杆菌O157引起的疫情。
方法
基于从大肠杆菌O157纯化的脂多糖（LPS）的ELISA和免疫印迹技术；诊断细菌学；血清分型和噬菌体分型；志贺毒素的DNA探针。
结果
126份血清中有29份含有针对大肠杆菌O157 LPS的抗体：10份来自儿童，3份来自工作人员，11份来自医院厨房工作人员。托儿所儿童的5名家长抗体呈阳性。来自其他医院工作人员和对照的64份血清不含有针对大肠杆菌O157 LPS的抗体。
结论
血清学检测在23%的受检血清中发现了感染大肠杆菌O157的证据。仅通过细菌学检测，仅能检测到1例大肠杆菌O157感染病例。血清学对于提供大肠杆菌O157感染的证据很有价值。
Zotero 插件