Garbay B, Boiron-Sargueil F, Shy M, Chbihi T, Jiang H, Kamholz J, Cassagne C
Laboratoire de Biogenèse Membranaire, UMR-CNRS 5544, Université Victor Ségalen Bordeaux 2, France.
J Neurochem. 1998 Oct;71(4):1719-26. doi: 10.1046/j.1471-4159.1998.71041719.x.
We studied the regulation of oleic acid synthesis in the PNS. During mouse postnatal development, the proportion of 18:1 rises in the sciatic nerve from 17% at 5 days of age to 33% at 25 days. However, this rise does not occur in the dysmyelinating mutant mouse trembler. In normal mouse development, the total stearoyl-CoA desaturase (SCD) activity measured in sciatic nerve homogenates is high during the first 3 weeks. Yet in trembler nerves, this SCD activity represents only 15% of normal values. Using the RT-PCR technique, we demonstrate that the SCD2 isoform is predominantly expressed in the PNS. Northern blot analysis showed that the mRNA levels for SCD2 parallel those of other specific myelin proteins in both normal mouse and trembler mutant development. Similar experiments in a rat demyelination-remyelination model confirmed that SCD2 mRNA levels are regulated in the PNS in a similar manner to myelin-specific proteins.
我们研究了周围神经系统（PNS）中油酸合成的调控。在小鼠出生后的发育过程中，坐骨神经中18:1的比例从5日龄时的17%上升至25日龄时的33%。然而，这种上升在脱髓鞘突变小鼠颤抖鼠中并未出现。在正常小鼠发育过程中，坐骨神经匀浆中测得的总硬脂酰辅酶A去饱和酶（SCD）活性在前三周较高。然而在颤抖鼠的神经中，这种SCD活性仅为正常值的15%。使用逆转录聚合酶链反应（RT-PCR）技术，我们证明SCD2亚型在周围神经系统中主要表达。Northern印迹分析表明，在正常小鼠和颤抖鼠突变体发育过程中，SCD2的mRNA水平与其他特定髓鞘蛋白的mRNA水平平行。在大鼠脱髓鞘-再髓鞘化模型中进行的类似实验证实，SCD2 mRNA水平在周围神经系统中的调控方式与髓鞘特异性蛋白相似。
