正常和震颤小鼠坐骨神经微粒体中硬脂酰辅酶A合成的测定

Assay of stearoyl-CoA synthesis in microsomes from normal and Trembler mouse sciatic nerves.

作者信息

Boiron F, Heape M A, Cassagne C

出版信息

Neurosci Lett. 1984 Jul 13;48(1):7-12. doi: 10.1016/0304-3940(84)90280-5.

Abstract

Using a new one-step technique which allows the characterization and a quantitative assay of long-chain acyl coenzyme A (acyl-CoA), the activity of the stearoyl-CoA synthetase was studied in the membrane fraction from sciatic nerves of normal and Trembler mice. A synthesis of acyl-CoAs was observed in both cases, but, whatever the experimental conditions, there was a two-fold increase of the specific activity of the ligase in the PNS of the Trembler mouse.

摘要

采用一种新的一步技术，该技术可对长链酰基辅酶A（酰基辅酶A）进行表征和定量测定，研究了正常小鼠和震颤小鼠坐骨神经膜组分中硬脂酰辅酶A合成酶的活性。在两种情况下均观察到酰基辅酶A的合成，但是，无论实验条件如何，震颤小鼠周围神经系统中连接酶的比活性都增加了两倍。