苋菜中两个编码甜菜碱醛脱氢酶的基因的分子特征。短期暴露于渗透胁迫或脱落酸下在叶片中的表达。

Molecular characterization of two genes encoding betaine aldehyde dehydrogenase from amaranth. Expression in leaves under short-term exposure to osmotic stress or abscisic acid.

作者信息

Legaria J, Rajsbaum R, Muñoz-Clares R A, Villegas-Sepúlveda N, Simpson J, Iturriaga G

机构信息

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Col. Chamilpa, 62210, Cuernavaca Mor., Mexico.

出版信息

Gene. 1998 Sep 18;218(1-2):69-76. doi: 10.1016/s0378-1119(98)00381-3.

DOI:10.1016/s0378-1119(98)00381-3

PMID:9751804

Abstract

A genomic clone (ahybadh4) and a cDNA (ahybadh17) both encoding betaine aldehyde dehydrogenase (BADH; EC 1.2.1.8) were isolated from the plant Amaranthus hypochondriacus L. The ahybadh4 gene extends 9 kilobases (kb) containing 15 exons with an open reading frame (ORF) of 501 amino acids (aa), a 1.3kb 5' untranslated region (UTR) and a 3' UTR of 0.3kb. The ahybadh17 cDNA encodes a BADH isoform of 500aa which contains 10aa substitutions with respect to AHYBADH4. Both encoded proteins share 98% identity at the amino acid level. Comparison of amaranth BADHs with other reported sequences showed high similarity. Analysis of ahybadh17 expression in amaranth leaves showed that mRNA and BADH protein are present in non-treated amaranth leaves and both transiently increased under short-term exposure to abscisic acid (ABA) and osmotic stress treatments.

摘要

从植物皱果苋（Amaranthus hypochondriacus L.）中分离出了一个基因组克隆（ahybadh4）和一个编码甜菜碱醛脱氢酶（BADH；EC 1.2.1.8）的cDNA（ahybadh17）。ahybadh4基因全长9千碱基（kb），包含15个外显子，开放阅读框（ORF）为501个氨基酸（aa），5'非翻译区（UTR）为1.3kb，3'UTR为0.3kb。ahybadh17 cDNA编码一个500aa的BADH同工型，与AHYBADH4相比有10个氨基酸替换。两种编码蛋白在氨基酸水平上有98%的同一性。将苋属BADH与其他已报道序列进行比较，显示出高度相似性。对皱果苋叶片中ahybadh17表达的分析表明，在未处理的皱果苋叶片中存在mRNA和BADH蛋白，并且在短期暴露于脱落酸（ABA）和渗透胁迫处理下两者均短暂增加。