Yamamoto Y, Ikeda K, Watanabe M, Shimomura A, Suzuki H, Oshima T, Imamura Y, Ohuchi K, Takasaka T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Int Arch Allergy Immunol. 1998 Sep;117(1):68-77. doi: 10.1159/000023992.
Adhesion molecules of microvascular endothelial cells play a key role in the inflammatory processes involved in nonallergic sinusitis. We investigated the cytokine-regulated expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1), and the effect of dexamethasone on these expressions in cultured human nasal microvascular endothelial cells (HNMEC). ICAM-1 was enhanced, and E-selectin and VCAM-1 were induced in a dose-dependent fashion following stimulation with IL-1beta or TNF-alpha. HNMEC differed from human umbilical vein endothelila cells in that (1) maximal upregulation of ICAM-1 expression induced by IL-1beta or TNF-alpha required more time (2) TNF-alpha was more potent than IL-1beta in VCAM-1 expression, and (3) dexamethasone inhibited the upregulation of E-selectin expression alone. These findings contribute to a better understanding of the characteristic features of leukocyte infiltration into inflamed tissue and the effect of glucocorticoid in nonallergic chronic sinusitis.
微血管内皮细胞的黏附分子在非过敏性鼻窦炎相关的炎症过程中起关键作用。我们研究了细胞因子调节的细胞间黏附分子-1（ICAM-1）、E-选择素和血管细胞黏附分子-1（VCAM-1）的表达，以及地塞米松对培养的人鼻微血管内皮细胞（HNMEC）中这些表达的影响。用IL-1β或TNF-α刺激后，ICAM-1表达增强，E-选择素和VCAM-1呈剂量依赖性诱导。HNMEC与人类脐静脉内皮细胞的不同之处在于：（1）IL-1β或TNF-α诱导的ICAM-1表达的最大上调需要更多时间；（2）TNF-α在VCAM-1表达方面比IL-1β更有效；（3）地塞米松单独抑制E-选择素表达的上调。这些发现有助于更好地理解白细胞浸润到炎症组织中的特征以及糖皮质激素在非过敏性慢性鼻窦炎中的作用。
