Piercey-Normore M D, Egger K N, Bérubé J A
Department of Biology, Memorial University of Newfoundland, St. John's, Canada.
Mol Phylogenet Evol. 1998 Aug;10(1):49-66. doi: 10.1006/mpev.1997.0485.
Armillaria is a genus of root infecting basidiomycetes, which includes nine North American biological species. Anonymous nucleotide sequences obtained from four different primer pairs were combined to produce a data set which was analyzed phylogenetically. The data indicated that randomly chosen sequences from the genome were capable of resolving the phylogenetic history of species of Armillaria and provided strong support for intraspecies clustering. NABS III and VII formed a significant monophyletic clade, with III being derived from the more broadly distributed NABS VII. Sequences of isolates of NABS V showed a high degree of variation. This variation may be an indication of recent sympatric speciation, with NABS IX and X diverging from a genetically diverse NABS V. NABS I formed a monophyletic clade despite the variation in geographic distance among the isolates. The position of NABS II as ancestral to NABS I was discussed. However, literature evidence favored divergence of NABS II from NABS I, while this study illustrated genetic similarity of NABS II with NABS VI. NABS VI was the most divergent of the North American species and represented the outgroup. A molecular clock of NABS Armillaria was proposed.
蜜环菌是一类感染根部的担子菌属，其中包括9个北美生物种。将从4种不同引物对获得的匿名核苷酸序列合并，以生成一个数据集，并进行系统发育分析。数据表明，从基因组中随机选择的序列能够解析蜜环菌物种的系统发育历史，并为种内聚类提供有力支持。NABS III和VII形成了一个显著的单系分支，其中III源自分布更广的NABS VII。NABS V分离株的序列显示出高度的变异性。这种变异可能表明近期存在同域物种形成，NABS IX和X从基因多样的NABS V分化而来。尽管分离株之间地理距离存在差异，但NABS I仍形成了一个单系分支。讨论了NABS II作为NABS I祖先的位置。然而，文献证据支持NABS II与NABS I的分化，而本研究表明NABS II与NABS VI具有遗传相似性。NABS VI是北美物种中差异最大的，代表了外类群。提出了NABS蜜环菌的分子钟。
