Wahlin T, Bloom G D, Danielsson A
Cell Tissue Res. 1976 Sep 1;171(4):425-35. doi: 10.1007/BF00220235.
Structural changes in the gallbladder epithelial cells of the mouse were studied following in vivo and in vitro stimulation of the gallbladder with the gastrointestinal hormone cholecystokinin-pancreozymin (CCK-PZ). Signs of increased secretory activity were observed within the first 2-3 min after hormone administration. At the ultrastructural level, best visualized with the PA-CrA-silver technique, granule discharge was observed, as was an overall increase in size of the granules. After prolonged in vitro incubation or repeated in vivo stimulation, there was an almost total depletion of secretory granules. This phenomenon is accompanied by an enhanced uptake of extracellular thorium dioxide by endocytotic vesicles at the apical cell surface. An exocytosis-endocytosis coupling mechanism may be important for membrane conservation in the gallbladder epithelial cells. The findings establish that the hormone CCK-PZ stimulates the secretion of glycoproteins from the mouse gallbladder epithelium.
在体外用胃肠激素胆囊收缩素-促胰酶素（CCK-PZ）刺激小鼠胆囊以及在体内刺激后，对小鼠胆囊上皮细胞的结构变化进行了研究。在给予激素后的最初2-3分钟内观察到分泌活动增强的迹象。在超微结构水平上，用PA-CrA-银技术能最清晰地观察到，发现有颗粒排出，并且颗粒的总体尺寸增大。在体外长时间孵育或在体内反复刺激后，分泌颗粒几乎完全耗尽。这种现象伴随着顶端细胞表面的内吞小泡对细胞外二氧化钍摄取的增强。胞吐-内吞偶联机制可能对胆囊上皮细胞的膜保存很重要。这些发现证实了激素CCK-PZ刺激小鼠胆囊上皮细胞分泌糖蛋白。
