Ravichandran D, Cooper A, Johnson C D
University Surgical Unit, Southampton General Hospital, UK.
Br J Surg. 1998 Sep;85(9):1201-5. doi: 10.1046/j.1365-2168.1998.00838.x.
The lithium salt of gamma-linolenic acid (Li-GLA) is growth inhibitory to pancreatic cancer cells in vitro and is reported to prolong the survival of patients with pancreatic cancer. The effect of Li-GLA on the growth of human pancreatic carcinoma in vivo is not known. In this study the effect of parenterally administered Li-GLA on the growth of human pancreatic carcinoma in nude mice was tested.
Pancreatic tumours were produced in nude mice by subcutaneous implantation of MIA PaCa-2 cells. This cell line is sensitive to Li-GLA in vitro. Mice were randomly treated with intraperitoneal, intravenous or intratumoral Li-GLA. Each group also had controls.
Both intravenous and intraperitoneal administration of Li-GLA had no significant effect on tumour growth or tumour phospholipid fatty acid composition. Intratumoral administration of Li-GLA was, however, associated with a significant antitumour effect.
Within the limitations of this tumour model, the benefit seen with intravenous Li-GLA in patients with pancreatic carcinoma cannot be explained by tumour growth inhibition. Local administration appears to be more effective than intravenous or intraperitoneal therapy.
γ-亚麻酸锂盐（Li-GLA）在体外对胰腺癌细胞具有生长抑制作用，据报道可延长胰腺癌患者的生存期。Li-GLA对人胰腺癌体内生长的影响尚不清楚。在本研究中，测试了经肠胃外给予Li-GLA对裸鼠体内人胰腺癌生长的影响。
通过皮下植入MIA PaCa-2细胞在裸鼠体内产生胰腺肿瘤。该细胞系在体外对Li-GLA敏感。将小鼠随机用腹膜内、静脉内或瘤内注射Li-GLA进行治疗。每组也设有对照。
静脉内和腹膜内给予Li-GLA对肿瘤生长或肿瘤磷脂脂肪酸组成均无显著影响。然而，瘤内注射Li-GLA具有显著的抗肿瘤作用。
在该肿瘤模型的局限性范围内，静脉注射Li-GLA对胰腺癌患者产生的益处无法通过肿瘤生长抑制来解释。局部给药似乎比静脉内或腹膜内治疗更有效。
