白细胞介素-1β导致大鼠溃疡复发时转化生长因子-β1和单核细胞趋化蛋白-1的mRNA水平升高。

Increased mRNA levels of transforming growth factor-beta1 and monocyte chemoattractant protein-1 in ulcer relapse caused by interleukin-1beta in rats.

作者信息

Tominaga K, Arakawa T, Watanabe T, Tanaka M, Takaishi O, Fujiwara Y, Fukuda T, Higuchi K, Kim S, Yamasaki K, Iwao H, Kobayashi K, Kuroki T

机构信息

Third Department of Internal Medicine, Osaka City University Medical School, Osaka, Japan.

出版信息

Dig Dis Sci. 1998 Sep;43(9 Suppl):134S-138S.

PMID:9753239

Abstract

This study investigated the mRNA expression of transforming growth factor-beta1 (TGF-beta1) and monocyte chemoattractant protein-1 (MCP-1) in rat gastric tissues in which ulcers had relapsed due to interleukin-1beta (IL-1beta) administration. Rats with healed ulcers were administered IL-1beta (1 microg/kg) and killed after 0, 12, 24, or 48 hr. Both TGF-beta1 and MCP-1 mRNA levels were increased in the scarred gastric tissues at 24 hr (fourfold), when ulcers had not relapsed. Furthermore, the expression of these genes also increased in the ulcerated gastric tissues at 48 hr (fivefold), when 90% of healed ulcers had relapsed. On the other hand, the number of macrophages that had infiltrated the scarred gastric tissues at 24 hr was two times higher than that at 0 hr. At 48 hr, the number of macrophages that had infiltrated gastric tissues in which ulcers had relapsed was similar to that at 24 hr. Thus, TGF-beta1 and MCP-1 may be implicated in the macrophage infiltration, thereby leading to ulcer relapse due to IL-1beta.

摘要

本研究调查了因给予白细胞介素-1β（IL-1β）而溃疡复发的大鼠胃组织中转化生长因子-β1（TGF-β1）和单核细胞趋化蛋白-1（MCP-1）的mRNA表达。对溃疡已愈合的大鼠给予IL-1β（1微克/千克），并在0、12、24或48小时后处死。在24小时时，当溃疡尚未复发时，瘢痕化胃组织中的TGF-β1和MCP-1 mRNA水平均升高（四倍）。此外，在48小时时，当90%已愈合的溃疡复发时，溃疡胃组织中这些基因的表达也增加（五倍）。另一方面，24小时时浸润瘢痕化胃组织的巨噬细胞数量比0小时时高出两倍。在48小时时，浸润溃疡已复发的胃组织的巨噬细胞数量与24小时时相似。因此，TGF-β1和MCP-1可能与巨噬细胞浸润有关，从而导致因IL-1β引起的溃疡复发。