Tominaga K, Arakawa T, Kim S, Iwao H, Kobayashi K
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Dig Dis Sci. 1997 Mar;42(3):616-25. doi: 10.1023/a:1018867630686.
This study was done to investigate the expression and localization of transforming growth factor-beta1 (TGF-beta1) in the gastric ulcerated tissues produced by acetic-acid during the healing process, by northern blot analysis and immunohistochemical technique. Ulcerated TGF-beta1 mRNA levels were significantly increased from days 3 to 18, in a similar manner to extracellular matrix proteins, and returned to control levels at the scarred phase. Immunoreactive TGF-beta1 was localized in epithelial cells beneath proliferative zone in intact tissues. In ulcerated tissues, TGF-beta1 was localized in macrophages in the ulcer bed and in fibroblasts or myofibroblasts in the granulation tissues. Treatment with prostaglandin E1 (PGE1) further stimulated ulcerated TGF-beta1 expression, being associated with the acceleration of gastric ulcer healing, while treatment with indomethacin reduced TGF-beta1 expression, being accompanied by the delayed ulcer healing. The combination of PGE1 and indomethacin reversed the indomethacin-induced decrease in ulcerated TGF-beta1. Thus, TGF-beta1 may be implicated in the acceleration of gastric ulcer healing.
本研究旨在通过Northern印迹分析和免疫组织化学技术，研究转化生长因子β1（TGF-β1）在乙酸所致胃溃疡组织愈合过程中的表达及定位。从第3天到第18天，溃疡组织中TGF-β1 mRNA水平显著升高，与细胞外基质蛋白的变化方式相似，并在瘢痕形成期恢复到对照水平。免疫反应性TGF-β1定位于完整组织中增殖区下方的上皮细胞。在溃疡组织中，TGF-β1定位于溃疡床的巨噬细胞以及肉芽组织中的成纤维细胞或肌成纤维细胞。前列腺素E1（PGE1）治疗进一步刺激溃疡组织中TGF-β1的表达，这与胃溃疡愈合加速相关，而吲哚美辛治疗则降低TGF-β1的表达，同时伴有溃疡愈合延迟。PGE1与吲哚美辛联合使用可逆转吲哚美辛所致溃疡组织中TGF-β1的降低。因此，TGF-β1可能与胃溃疡愈合加速有关。
