Young H M, Torihashi S, Ciampoli D, Sanders K M
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Australia.
Gastroenterology. 1998 Oct;115(4):898-908. doi: 10.1016/s0016-5085(98)70262-8.
BACKGROUND & AIMS: Enteric neurons in the murine intestine express stem cell factor (SCF), which may provide an important signal in the development of the interstitial cells of Cajal (ICC). Our aim was to identify the subpopulation(s) of myenteric neurons that express SCF.
Myenteric plexus preparations from postnatal SCF-lacZ mice were processed for beta-galactosidase histochemistry followed by immunohistochemistry.
Approximately 60% of the nitric oxide synthase-immunoreactive neurons, which projected to myenteric ganglia and to circular muscle, expressed SCF, and more than 80% of the calbindin-immunoreactive neurons, which projected exclusively to myenteric ganglia, expressed SCF. A small subpopulation of calretinin-immunoreactive neurons expressed SCF transiently. Many of the remainder of SCF-expressing neurons were choline acetyltransferase immunoreactive, but their projections are unknown.
SCF-expressing neurons that project within the myenteric plexus may be an important source of SCF for the development of Kit-expressing ICC at this level. The only possible neuronal source of SCF for the ICC of the deep muscular plexus is a subpopulation of nitric oxide synthase-immunoreactive neurons.
小鼠肠道中的肠神经元表达干细胞因子（SCF），这可能在Cajal间质细胞（ICC）的发育中提供重要信号。我们的目的是确定表达SCF的肌间神经元亚群。
对出生后SCF-lacZ小鼠的肌间神经丛标本进行β-半乳糖苷酶组织化学处理，随后进行免疫组织化学处理。
投射至肌间神经节和环行肌的一氧化氮合酶免疫反应性神经元中约60%表达SCF，而仅投射至肌间神经节的钙结合蛋白免疫反应性神经元中超过80%表达SCF。一小部分钙视网膜蛋白免疫反应性神经元短暂表达SCF。其余表达SCF的神经元中许多是胆碱乙酰转移酶免疫反应性的，但其投射情况未知。
在肌间神经丛内投射的表达SCF的神经元可能是该水平表达Kit的ICC发育过程中SCF的重要来源。深部肌丛ICC的SCF唯一可能的神经元来源是一氧化氮合酶免疫反应性神经元亚群。
