Sharan R N, Schneeweiss F H, Saikia J R, Feinendegen L E
Department of Biochemistry, North-Eastern Hill University, Shillong, India.
Indian J Biochem Biophys. 1998 Apr;35(2):97-102.
Poly-ADP-ribosylation of cellular proteins is involved with radiation induced damage and its repair. It has been observed that suspension of human kidney T1-cells in vitro attained elevated levels of poly-ADP-ribosylation due to experimental manipulations necessary for preparation of single cell suspension from monolayer cell cultures. These cells in suspension were exposed to various doses of gamma-rays with or without subsequent repair incubation. The PADPR of histones H3, H1 and H2B increased with increasing dose of radiation and decreased after 90 min or repair incubation. Concomitant with these changes, the affinity of histones to DNA in chromatin reduced immediately after irradiation. Normal affinity was reestablished after post-irradiation repair incubation. The results indicate that induction of poly-ADP-ribosylation of histone proteins by radiation and by manipulations to prepare single cell suspension involved different cellular components.
细胞蛋白质的多聚 ADP 核糖基化与辐射诱导的损伤及其修复有关。据观察，由于从单层细胞培养物制备单细胞悬液所需的实验操作，人肾 T1 细胞在体外悬浮时多聚 ADP 核糖基化水平升高。将这些悬浮细胞暴露于不同剂量的γ射线，有无后续修复孵育。组蛋白 H3、H1 和 H2B 的多聚 ADP 核糖基化随辐射剂量增加而增加，并在 90 分钟或修复孵育后降低。伴随这些变化，染色质中组蛋白与 DNA 的亲和力在照射后立即降低。照射后修复孵育后恢复正常亲和力。结果表明，辐射和制备单细胞悬液的操作诱导组蛋白的多聚 ADP 核糖基化涉及不同的细胞成分。
