Kim H S, Lee E H, Sungjoo K Y, Joo C K
Department of Ophthalmology, Catholic University Medical College, Seoul, Korea.
Korean J Ophthalmol. 1998 Jun;12(1):14-8. doi: 10.3341/kjo.1998.12.1.14.
The etiology of uveitis, a general term for inflammatory disorders of the uveal tract, has not been clarified. The purpose of this study is to investigate the immunopathogenesis during experimental uveitis induced by bovine melanin associated antigen (BMAA). Experimental melanin-induced uveitis (EMIU) was induced in male Lewis rats by injection of BMAA and delayed type hypersensitivity (DTH) testing was performed. Fluorescence-activated cell sorting (FACS) analysis was used to examine expression of cell adhesion molecules with antibodies to ICAM-1 and LFA-1. BMMA-induced uveitis resembled human AAU, with onset and peak at approximately 14 and 21 days after immunization, respectively. Signs of uveitis disappeared by 4 weeks postimmunization. Histologic study revealed major infiltration of the iris and ciliary body by numerous inflammatory cells and minor infiltration of the choroid. DTH testing showed that ears injected with antigen were more swollen than non-injected ears. FACS analysis demonstrated that ICAM-1 and LFA-1 expression increased during EMIU, with ICAM-1 expression higher than that of LFA-1. In conclusion, BMAA is uveitogenically active in Lewis rats. Immunopathogenesis appeared to be mediated by cell expressing ICAM-1 and LFA-1.
葡萄膜炎是葡萄膜炎症性疾病的统称，其病因尚未明确。本研究旨在探讨牛黑色素相关抗原（BMAA）诱导的实验性葡萄膜炎的免疫发病机制。通过注射BMAA在雄性Lewis大鼠中诱导实验性黑色素诱导性葡萄膜炎（EMIU），并进行迟发型超敏反应（DTH）检测。使用荧光激活细胞分选（FACS）分析，用抗ICAM-1和LFA-1抗体检测细胞黏附分子的表达。BMMA诱导的葡萄膜炎类似于人类急性前葡萄膜炎，分别在免疫后约14天和21天发病并达到高峰。免疫后4周葡萄膜炎症状消失。组织学研究显示，虹膜和睫状体有大量炎症细胞浸润，脉络膜有少量浸润。DTH检测表明，注射抗原的耳朵比未注射的耳朵肿胀更明显。FACS分析表明，在EMIU期间ICAM-1和LFA-1表达增加，ICAM-1表达高于LFA-1。总之，BMAA在Lewis大鼠中具有葡萄膜炎致病活性。免疫发病机制似乎由表达ICAM-1和LFA-1的细胞介导。
