用氯化锌固定糊剂治疗高危人类黑色素瘤的实验原理。在小鼠黑色素瘤模型中对肿瘤攻击的抵抗力增强。
Experimental rationale for treatment of high-risk human melanoma with zinc chloride fixative paste. Increased resistance to tumor challenge in murine melanoma model.
作者信息
Kalish R S, Wood J A, Siegel D M, Kaye V N, Brooks N A
机构信息
Department of Dermatology, State University of New York, Stony Brook, USA.
出版信息
Dermatol Surg. 1998 Sep;24(9):1021-5. doi: 10.1111/j.1524-4725.1998.tb04296.x.
BACKGROUND
Fixed-tissue micrographic surgery (Mohs) of melanoma has been shown by retrospective analysis to improve 5-year survival.
OBJECTIVES
To determine whether zinc chloride fixative paste acts as an immune adjuvant to increase host resistance to melanoma.
METHODS
We performed a murine study using the poorly immunogenic B16 melanoma of C57Bl6J mice, and the more immunogenic K1735p melanoma of C3H/HeN mice. Tumors were treated with zinc chloride paste and excised 24 hours later (Group 1), or simply excised (Group 2). Mice were challenged 7 days later with injection of melanoma cells at a distant site, and tumor growth in this second site was followed.
RESULTS
K1735p melanomas developed at the challenge site in 69% of mice treated with excision versus 32% of mice treated with zinc chloride fixation (P < 0.025). Development of B16 melanoma was not altered by zinc chloride fixation.
CONCLUSION
Zinc chloride fixation of the more immunogenic K1735p melanoma increased resistance to subsequent tumor challenge, suggesting that zinc chloride fixative paste acts as an immune adjuvant.
背景
回顾性分析表明，黑色素瘤的固定组织显微外科手术（莫氏手术）可提高5年生存率。
目的
确定氯化锌固定糊剂是否作为免疫佐剂来增强宿主对黑色素瘤的抵抗力。
方法
我们使用免疫原性较差的C57Bl6J小鼠的B16黑色素瘤和免疫原性较强的C3H/HeN小鼠的K1735p黑色素瘤进行了一项小鼠研究。用氯化锌糊剂处理肿瘤并在24小时后切除（第1组），或直接切除（第2组）。7天后，在远处部位注射黑色素瘤细胞对小鼠进行攻击，并观察第二个部位的肿瘤生长情况。
结果
在接受切除治疗的小鼠中，69%在攻击部位出现了K1735p黑色素瘤，而接受氯化锌固定治疗的小鼠中这一比例为32%（P < 0.025）。氯化锌固定对B16黑色素瘤的发生没有影响。
结论
对免疫原性较强的K1735p黑色素瘤进行氯化锌固定可增加对后续肿瘤攻击的抵抗力，这表明氯化锌固定糊剂起到了免疫佐剂的作用。
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