Pratesi R, Gandolfi L, Friedman H, Farage L, de Castro C A, Catassi C
Dept. of Pediatrics, Brasilia University, Brazil.
Scand J Gastroenterol. 1998 Aug;33(8):817-21. doi: 10.1080/00365529850171468.
Our objective was to determine the possible presence of IgA antibodies directed against human central nervous system (CNS) structures in sera from coeliac disease (CD) patients.
Serum samples were collected from 4 patients with active CD on a gluten-containing diet, 11 biopsy-proven CD patients on a gluten-free diet (GFD), and 52 non-coeliac gastrointestinal controls. In all patients IgA antigliadin antibody (AGA) titres were determined with enzyme-linked immunoassay (ELISA), and IgA antiendomysium antibodies (EMA) with indirect immunofluorescence on human umbilical cord. Cryostat sections of human brain occipital cortex were incubated with the patients' sera and subsequently labelled with anti-human IgA fluorescein conjugate.
All sera from patients with active CD on a gluten-containing diet yielded positive results in both the IgG-AGA and EMA test and in indirect immunofluorescence on brain tissue, disclosing a strong fluorescence over blood-vessels structures. All sera from CD patients on a GFD and from non-coeliac gastrointestinal controls gave a negative result on both the EMA test and the immunofluorescence reaction on human brain.
Sera from patients with active CD contain IgA antibodies that react with human brain vessel structures, giving intense fluorescence. These antibodies are not present in sera from coeliac patients on a GFD or non-coeliac controls. This finding might be involved in the abnormal nervous system manifestations frequently described in association with coeliac disease.
我们的目的是确定乳糜泻（CD）患者血清中是否可能存在针对人类中枢神经系统（CNS）结构的IgA抗体。
收集4例食用含麸质饮食的活动性CD患者、11例经活检证实的食用无麸质饮食（GFD）的CD患者以及52例非乳糜泻胃肠道对照者的血清样本。所有患者均采用酶联免疫吸附测定（ELISA）法测定IgA抗麦醇溶蛋白抗体（AGA）滴度，采用人脐带间接免疫荧光法测定IgA抗肌内膜抗体（EMA）。将人脑枕叶皮质的冰冻切片与患者血清孵育，随后用抗人IgA荧光素偶联物标记。
所有食用含麸质饮食的活动性CD患者的血清在IgG-AGA和EMA检测以及脑组织间接免疫荧光检测中均呈阳性结果，显示血管结构上有强烈荧光。所有食用GFD的CD患者血清和非乳糜泻胃肠道对照者血清的EMA检测和人脑免疫荧光反应均为阴性结果。
活动性CD患者的血清中含有与人类脑血管结构发生反应并产生强烈荧光的IgA抗体。这些抗体在食用GFD的乳糜泻患者血清或非乳糜泻对照者血清中不存在。这一发现可能与乳糜泻常伴有的异常神经系统表现有关。
