Müller-Ostermeyer F, Ebert M, Malfertheiner P, Schubert W
Institute of Medical Neurobiology, Dept. of Gastroenterology, Hepatology, and Infectious Diseases, Otto-von-Guericke-University of Magdeburg, Germany.
Scand J Gastroenterol. 1998 Aug;33(8):872-9. doi: 10.1080/00365529850171558.
T lymphocytes play a central role in the immune response to cancer, with specific T-cell reactivity provided by the T-cell receptor (TCR) alphabeta-chain heterodimer. Whereas human pancreatic adenocarcinoma is characterized by a massive infiltration of T lymphocytes, to date no analysis of the TCR Valpha-gene expression of the tumor-infiltrating lymphocytes in pancreatic cancer has been performed.
Using reverse transcriptase polymerase chain reaction (RT-PCR) followed by dot blot hybridization, we determined the TCR alpha-chain repertoire at the mRNA level in pancreatic carcinoma and compared our findings with the TCR Valpha repertoire in the normal pancreas and chronic pancreatitis.
A heterogeneous lowly restricted TCR Valpha repertoire was observed in pancreatic carcinomas, different from the TCR Valpha repertoire in chronic pancreatitis.
Pancreas-infiltrating T cells show a distinct TCR Valpha gene expression profile in normal pancreas, chronic pancreatitis, and pancreatic cancer.
T淋巴细胞在癌症免疫反应中起核心作用，由T细胞受体（TCR）αβ链异二聚体提供特异性T细胞反应性。尽管人类胰腺腺癌的特征是T淋巴细胞大量浸润，但迄今为止尚未对胰腺癌中肿瘤浸润淋巴细胞的TCR Vα基因表达进行分析。
采用逆转录聚合酶链反应（RT-PCR），随后进行点杂交，我们在mRNA水平测定了胰腺癌中TCR α链库，并将我们的发现与正常胰腺和慢性胰腺炎中的TCR Vα库进行比较。
在胰腺癌中观察到异质性的低限制性TCR Vα库，不同于慢性胰腺炎中的TCR Vα库。
胰腺浸润性T细胞在正常胰腺、慢性胰腺炎和胰腺癌中表现出独特的TCR Vα基因表达谱。
