Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China.
Department of Gastroenterology, Nanjing Medical University Affiliated Drum Tower Clinical Medical College, Nanjing, China.
J Cell Mol Med. 2021 Apr;25(8):3991-4000. doi: 10.1111/jcmm.16358. Epub 2021 Mar 8.
Pancreatic cancer (PC) has been the fourth cancer-related death worldwide, diagnosed at an unresectable stage due to its rapid progression and few symptoms of this disease at early stages. The aim of this study was to determine the association between the diversity of T-cell receptor (TCR) repertoire and clinicopathological characteristics of patients with PC and other benign pancreatic diseases. In order to make a comprehensive analysis the TCR repertoire, high-throughput sequencing was used to differentiate complementarity determining region 3 (CDR3) of the TCR β chain in peripheral blood samples from 3 PC, 3 chronic pancreatitis, 3 pancreatic cystic lesions and 3 pancreatic neuroendocrine tumour patients. We found that there were significant differences related to TCR repertoire between PC and other pancreatic diseases, and PC is a relatively immunosuppressive tumour. Changes of peripheral TCR repertoire may be used to predict the progression of PC and the response to immunotherapy. And there may exist novel-specific antigens in PC patients which could be used to design targeting immunotherapy in the nearly future.
胰腺癌（PC）是全球第四大癌症相关死因，由于其快速进展和早期疾病症状很少，大多数患者在确诊时已无法进行手术切除。本研究旨在确定 T 细胞受体（TCR）多样性与 PC 患者和其他良性胰腺疾病的临床病理特征之间的关联。为了全面分析 TCR 库，我们使用高通量测序技术区分了 3 例 PC、3 例慢性胰腺炎、3 例胰腺囊性病变和 3 例胰腺神经内分泌肿瘤患者外周血样本中 TCRβ 链的互补决定区 3（CDR3）。我们发现 PC 与其他胰腺疾病之间的 TCR 库存在显著差异，PC 是一种相对免疫抑制性肿瘤。外周 TCR 库的变化可能用于预测 PC 的进展和免疫治疗的反应。并且在 PC 患者中可能存在新的特异性抗原，这些抗原可能在不久的将来用于设计针对免疫治疗的方案。
