腺病毒介导的转基因在小鼠骨骼肌纤维中的纤维类型依赖性表达。

Fiber-type-dependent expression of adenovirus-mediated transgene in mouse skeletal muscle fibers.

作者信息

Hagiwara Y, Ishii A, Nonaka I, Kikuchi T, Takeda S

机构信息

National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.

出版信息

Acta Neuropathol. 1998 Sep;96(3):228-32. doi: 10.1007/s004010050888.

DOI:10.1007/s004010050888

PMID:9754954

Abstract

We show the efficient transduction and expression of the lacZ gene in the skeletal muscle of adult C57BL/10ScSn mice after adenovirus-mediated gene transfer. Of the myofibers in the tibialis anterior muscle 62% were beta-galactosidase positive after injection of the lacZ gene under the control of the chicken beta-actin promoter and the cytomegalovirus enhancer. The transduced gene was preferentially expressed in type IIA and IIX fibers, which were richer in oxidative enzymes than type IIB fibers.

摘要

我们展示了腺病毒介导的基因转移后，lacZ基因在成年C57BL/10ScSn小鼠骨骼肌中的高效转导和表达。在鸡β-肌动蛋白启动子和巨细胞病毒增强子的控制下注射lacZ基因后，胫前肌中62%的肌纤维β-半乳糖苷酶呈阳性。转导基因优先在IIA型和IIX型纤维中表达，这两种纤维比IIB型纤维富含更多氧化酶。