Goldberg R N
Clin Chem. 1976 Oct;22(10):1586-91.
Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by the spectrophotometric hexokinase/glucose-6-phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two methods. A discussion of the basis of the microcalorimetric measurements is presented.
我报告了对五个血清参考样品中葡萄糖浓度进行微量量热法测量的详细系列结果。所采用的方法包括：(a) 在实际分析介质中进行校准；(b) 针对由于己糖激酶酶非特异性导致的干扰进行校正。将微量量热法的结果与通过分光光度法己糖激酶/葡萄糖-6-磷酸脱氢酶法和同位素稀释质谱法获得的初步分析结果进行了比较。在大多数情况下，微量量热法的结果似乎与后两种方法获得的结果一致。本文还对微量量热法测量的基础进行了讨论。
