Vesely M J, Exon D J, Clark J E, Foresti R, Green C J, Motterlini R
Vascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, Middlesex, HA1 3UJ, United Kingdom.
Am J Physiol. 1998 Oct;275(4):C1087-94. doi: 10.1152/ajpcell.1998.275.4.C1087.
The heat shock protein heme oxygenase-1 (HO-1) is regulated by a variety of physiological and pharmacological factors. In skeletal muscle tissue, HO-1 has been shown to be induced only by exercise and electrical stimulation in vivo. Both hemin and sodium nitroprusside (SNP) are potent inducers of HO-1 in other tissues. In this study, we examined the effects of these two agents on HO-1 induction in L6.G8 rat skeletal myoblast cells. Hemin and SNP increased cellular heme oxygenase activity in both a time- and concentration-dependent manner. Increases in the HO-1 mRNA level and protein expression accompanied changes in heme oxygenase activity. The ability of SNP to induce HO-1 in L6.G8 cells was reduced by coincubation with hydroxocobalamin, a known nitric oxide (NO) scavenger, suggesting that NO itself may be involved in HO-1 gene stimulation. These results indicate that HO-1 expression is sensitive to both hemin and SNP in skeletal myoblast cells and may indicate an important regulatory mechanism of heme catabolism in skeletal muscle tissue.
热休克蛋白血红素加氧酶-1（HO-1）受多种生理和药理因素调控。在骨骼肌组织中，研究表明HO-1仅在体内运动和电刺激时被诱导。血红素和硝普钠（SNP）在其他组织中都是HO-1的有效诱导剂。在本研究中，我们检测了这两种试剂对L6.G8大鼠骨骼肌成肌细胞中HO-1诱导的影响。血红素和SNP均以时间和浓度依赖性方式增加细胞血红素加氧酶活性。HO-1 mRNA水平和蛋白表达的增加伴随着血红素加氧酶活性的变化。与已知的一氧化氮（NO）清除剂羟钴胺素共同孵育可降低SNP在L6.G8细胞中诱导HO-1的能力，这表明NO本身可能参与HO-1基因的刺激。这些结果表明，HO-1表达在骨骼肌成肌细胞中对血红素和SNP均敏感，这可能表明骨骼肌组织中血红素分解代谢的一种重要调节机制。
