Nakhostin-Roohi Babak, Niknam Zahra, Vaezi Nasrin, Mohammadi Sadollah, Bohlooli Shahab
Department of Exercise Physiology, Ardabil Branch, Islamic Azad University, Iran.
Department of Exercise Physiology, University of Mohaghegh-Ardabili, Ardabil, Iran.
Iran J Pharm Res. 2013 Fall;12(4):845-53.
Methylsulfonylmethane (MSM) is a sulfur-containing compound commonly found in diet and known to reduce oxidative stress. This trial was conducted to determine whether single dose supplementation with MSM attenuates post-exercise oxidative stress in healthy untrained young men. Sixteen untrained men volunteered for this study. Participants were randomized in a double-blind placebo-controlled fashion into 2 groups: Methylsulfonylmethane (MSM) (n = 8) and placebo (n = 8). The participants took supplementation or placebo before running on treadmill for 45 min at 75% VO2max. The MSM supplementation was prepared in water as 100 mg/ kg body weight. The placebo group received water. Serum Malondealdehyde (MDA), uric acid, bilirubin, protein carbonyl (PC) and plasma vitamin E levels were determined as the markers of oxidative stress. Plasma GSH (reduced Glutathione) and total antioxidant capacity (TAC) were measured as markers of plasma antioxidant system. MSM supplementation successfully lowered serum PC 2 and 24 h after exercise. Plasma TAC in MSM group was higher at 24 h after exercise. Serum level of uric acid and bilirubin were significantly low immediately after exercise in MSM supplemented group. There was no significant difference between groups in terms of plasma GSH level. These results complement earlier studies showing anti-oxidant effect of MSM and suggest that single dose oral supplementation with MSM lowers exercise induced oxidative stress in healthy untrained young men, but is not adequate to significantly affect plasma GSH level.
甲基磺酰甲烷（MSM）是一种常见于饮食中的含硫化合物，已知其能减轻氧化应激。本试验旨在确定单剂量补充MSM是否能减轻健康未受过训练的年轻男性运动后的氧化应激。16名未受过训练的男性自愿参加本研究。参与者以双盲安慰剂对照的方式随机分为两组：甲基磺酰甲烷（MSM）组（n = 8）和安慰剂组（n = 8）。参与者在以75%最大摄氧量在跑步机上跑步45分钟前服用补充剂或安慰剂。MSM补充剂以每公斤体重100毫克的剂量溶于水中配制。安慰剂组则服用水。测定血清丙二醛（MDA）、尿酸、胆红素、蛋白质羰基（PC）和血浆维生素E水平作为氧化应激的标志物。测定血浆谷胱甘肽（还原型谷胱甘肽）和总抗氧化能力（TAC）作为血浆抗氧化系统的标志物。补充MSM成功降低了运动后2小时和24小时的血清PC水平。MSM组运动后24小时的血浆TAC较高。补充MSM组运动后即刻血清尿酸和胆红素水平显著降低。两组间血浆谷胱甘肽水平无显著差异。这些结果补充了早期显示MSM具有抗氧化作用的研究，并表明单剂量口服补充MSM可降低健康未受过训练的年轻男性运动诱导的氧化应激，但不足以显著影响血浆谷胱甘肽水平。
