Kykstra S L, Lawrence J D
Clin Chem. 1976 Nov;22(11):1896-9.
We compared three commercially available kits for use in determining triglycerides (triacylglycerols) in serum, by different methods, for accuracy and precision, with use of pooled sera of low, normal, and high triglyceride concentrations. Accuracy varied from -17% to +16% of the independently determined value, depending on the method and triglyceride concentration. In general, the Calbiochem method was the most nearly accurate, and only it met Center for Disease Control criteria for precision at all three triglyceride concentrations. The relationships of mean values to the suggested normal range for each method varied, some significant differences being found. Our data demonstrate the importance of a correlation between the independently determined value and the normal range for the method used and stresses the need for normal-range values to accompany all patients' reports.
我们比较了三种市售试剂盒,它们采用不同方法测定血清中的甘油三酯(三酰甘油),以评估其准确性和精密度,使用的是低、正常和高甘油三酯浓度的混合血清。根据方法和甘油三酯浓度的不同,准确性在独立测定值的-17%至+16%之间变化。总体而言,Calbiochem方法最为准确,只有它在所有三种甘油三酯浓度下均符合疾病控制中心的精密度标准。每种方法的平均值与建议正常范围的关系各不相同,发现了一些显著差异。我们的数据表明,独立测定值与所用方法的正常范围之间进行相关性分析非常重要,并强调所有患者报告都应附带正常范围值。