[肾移植中全血中环孢素水平的测定]
[Measuring cyclosporine levels in whole blood in kidney transplantation].
作者信息
Bottini P V, Alves-Cunha F A, Moda M A, Souza M I, Garlipp C R
机构信息
Departamento de Patologia Clínica, Faculdade de Ciências Médicas, UNICAMP, Campinas, SP.
出版信息
Rev Assoc Med Bras (1992). 1998 Jul-Sep;44(3):176-8. doi: 10.1590/s0104-42301998000300003.
BACKGROUND
Cyclosporin A is a potent immunosuppressive drug effective in combatting rejection following organ transplantation. In na effort to replace a radioimmunoassay (RIA) for whole blood determination of cyclosporine (Cya) we compared RIA with fluorescence polarization immunoassay (FPIAm).
METHOD
65 blood samples were analysed from kidney transplanted patients. The samples were collected into tubes containing EDTA as anticoagulant and analysed by RIA and FPIAm.
RESULTS
The statistical analysis revealed a difference between both methods (p < 0.05). The linear-regression comparison of Cya concentration measured by RIA and FPIAm showed the following relationship: Cya(FPIAm) = 1.06 x Cya(RIA) + 5.8 (r = 0.9817).
CONCLUSION
We conclude that FPIAm provides na alternative method for measuring cyclosporine in whole blood with the added advantages of being reasonably rapid, precise and easy to perform.
背景
环孢素A是一种有效的免疫抑制药物，对器官移植后抵抗排斥反应有效。为了替代用于全血中环孢素（Cya）测定的放射免疫分析（RIA），我们将RIA与荧光偏振免疫分析（FPIAm）进行了比较。
方法
对65例肾移植患者的血样进行分析。将血样采集到含有乙二胺四乙酸作为抗凝剂的试管中，并通过RIA和FPIAm进行分析。
结果
统计分析显示两种方法之间存在差异（p < 0.05）。通过RIA和FPIAm测量的Cya浓度的线性回归比较显示出以下关系：Cya（FPIAm）= 1.06×Cya（RIA）+ 5.8（r = 0.9817）。
结论
我们得出结论，FPIAm为全血中环孢素的测量提供了一种替代方法，具有相当快速、精确且易于操作的额外优点。
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