David-Neto E, Ballarati C A, Freitas O J, Lemos F C, Nahas W C, Arap S, Kalil J
Hospital Sírio Libanes.
Rev Hosp Clin Fac Med Sao Paulo. 2000 Nov-Dec;55(6):207-12. doi: 10.1590/s0041-87812000000600003.
Evaluation of Cyclosporin A (CyA) blood concentration is imperative in solid organ transplantation in order to achieve maximal immunosuppression with the least side effects. We compared the results of whole blood concentrations of CyA in 50 blood samples simultaneously evaluated by the fluorescent polarization immune assay (TDx) and the enzymatic competitive immune assay (EMIT 2000). There was a strong correlation between both kits for any range of CyA blood concentration (R=0.99, p<0.001). The within-run and between-days coefficient of variation were less than 4% for both assays. The cost for each CyA measurement was 50% lower for the EMIT assay when compared to the TDx assay. We concluded that the EMIT is as accurate as the TDx in measuring CyA blood concentration and has the advantage of a lower cost, as well as the possibility of widespread access to the EMIT methodology in contrast to the TDx equipment, allowing the laboratory to perform several routines within a working day.
在实体器官移植中，评估环孢素A（CyA）血药浓度对于以最小的副作用实现最大程度的免疫抑制至关重要。我们比较了通过荧光偏振免疫分析法（TDx）和酶竞争性免疫分析法（EMIT 2000）同时评估的50份血样中CyA全血浓度的结果。对于任何范围的CyA血药浓度，两种试剂盒之间都存在很强的相关性（R = 0.99，p < 0.001）。两种检测方法的批内和批间变异系数均小于4%。与TDx检测法相比，EMIT检测法每次检测CyA的成本低50%。我们得出结论，在测量CyA血药浓度方面，EMIT与TDx一样准确，并且具有成本较低的优势，而且与TDx设备相比，EMIT方法更有可能广泛应用，使实验室能够在一个工作日内完成多项常规检测。
