放射免疫分析法与薄层色谱法和气相-液相色谱法在检测人尿中美沙酮方面的比较。

Radioimmunuassay compared to thin-layer and gas--liquid chromatography for detecting methadone in human urine.

作者信息

Roerig D L, Wang R I, Mueller M M, Lewand D L, Adams S M

出版信息

Clin Chem. 1976 Nov;22(11):1915-8.

Abstract

Radioimmunoassay was compared to thin-layer and gas--liquid chromatographic methods for detection of methadone in the urine of patients undergoing methadone maintenance therapy as treatment of heroin abuse. With urine samples known to contain methadone, 84% were positive by thin-layer chromatography as compared to 99% positives by the other two methods. This difference is attributed to the difference in sensitivity of the three methods. All three methods gave consistently positive results with urine samples from patients receiving 25 mg of methadone per day or more. With smaller daily doses the percentage of positive results obtained with thin-layer chromatography decreased. Analysis of urine samples not containing methadone showed no incidence of cross reaction of other drugs with the methadone radioimmunoassay. The methadone radioimmunoassay appears to be both sensitive and reliable; however, certain other factors limit its use as a primary screening method.

摘要

将放射免疫分析法与薄层色谱法和气液色谱法进行了比较，以检测接受美沙酮维持疗法治疗海洛因成瘾患者尿液中的美沙酮。对于已知含有美沙酮的尿液样本，薄层色谱法的阳性检出率为84%，而其他两种方法的阳性检出率为99%。这种差异归因于三种方法灵敏度的不同。对于每日服用25毫克或更多美沙酮患者的尿液样本，三种方法均能持续给出阳性结果。每日剂量较小时，薄层色谱法获得的阳性结果百分比会下降。对不含美沙酮的尿液样本分析表明，其他药物与美沙酮放射免疫分析法不存在交叉反应。美沙酮放射免疫分析法似乎既灵敏又可靠；然而，某些其他因素限制了其作为主要筛查方法的应用。