Nicolau G, Van Lear G, Kaul B, Davidow B
Clin Chem. 1977 Sep;23(9):1640-3.
We describe a micromethod for determining as little as 0.1 microgram of morphine per liter of human urine. The procedure is about 20-fold more sensitive than are currently used gas-liquid chromatographic methods. It is particularly suitable for use as a confirmatory method for large-scale radioimmunoassay screening tests. The procedure involves extraction of morphine from urine and analysis of its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture detection. Morphine can be determined by this procedure over a range of 0.1-200 microgram/liter of urine, 2 pg of the pure derivative being the lowest amount detectable. Urine samples from patients receiving methadone were analyzed by thin-layer chromatography, radioimmunoassay, and our procedure. The results by our procedure agreed well with those obtained by radioimmunoassay.
我们描述了一种微量方法，可测定每升人尿中低至0.1微克的吗啡。该方法的灵敏度比目前使用的气液色谱法高约20倍。它特别适合用作大规模放射免疫分析筛选试验的确认方法。该方法包括从尿液中提取吗啡，并通过带有电子捕获检测的气液色谱法分析其七氟丁酰衍生物。通过该方法可在0.1 - 200微克/升的尿样范围内测定吗啡，2皮克纯衍生物是可检测的最低量。对接受美沙酮治疗的患者的尿样进行了薄层色谱法、放射免疫分析法和我们的方法分析。我们的方法所得结果与放射免疫分析法所得结果非常吻合。
