Rhee J K, Kim H C, Eun G S
Department of Parasitology, Veterinary School and Bio-Safety Research Institute, Chonbuk National University, Chonju, Korea.
Korean J Parasitol. 1998 Sep;36(3):171-81. doi: 10.3347/kjp.1998.36.3.171.
A total of nine Korean native kids and two Corriedale lambs, 1-20 days old, were each inoculated per os with a single dose of 2 x 10(7) oocysts of Cryptosporidium muris (strain MCR) originated from mice to elucidate the kinetics and developmental stages of the coccidium in small ruminants. Irrespective of host's age, the prepatent period for both animals ranged from 19 to 35 days (28.1 days, on the average) and the patent period 16-85 days (47.8 days), and the total oocyst outputs showed enormous differences. Infection with greater numbers of oocyst outputs was not ordinarily established by transmission experiments. Oocysts discharged from the kids retained their infectivity by the mouse titration method. The immunogenicity of the coccidium and oocyst reproduction were proven by challenge infection and administration of prednisolone acetate, respectively. All the developmental stages of the coccidium in parasitophorous vacuoles were found by transmission electron microscopy in the pits of the gastric glands of a kid inoculated with oocysts and then necropsied on day 44 postinoculation. It indicated the full course of the host-parasite relationship in kids and lambs as well as mice.
为了阐明小型反刍动物体内鼠隐孢子虫（MCR株）的动力学和发育阶段，给9只韩国本土幼童和2只1至20日龄的考力代羔羊经口接种了单剂量源自小鼠的2×10⁷个鼠隐孢子虫卵囊。不论宿主年龄大小，两种动物的潜隐期为19至35天（平均28.1天），排虫期为16至85天（平均47.8天），且总的卵囊排出量显示出巨大差异。通过传播实验通常无法建立卵囊排出量更多的感染。通过小鼠滴定法证明，幼童排出的卵囊具有感染力。分别通过攻击感染和给予醋酸泼尼松龙证明了隐孢子虫的免疫原性和卵囊繁殖能力。在接种卵囊后第44天剖检的一只幼童的胃腺凹窝中，通过透射电子显微镜观察到了隐孢子虫在寄生泡内的所有发育阶段。这表明了幼童、羔羊以及小鼠体内宿主 - 寄生虫关系的全过程。
